Frequently Asked Questions
What is the isoelectric point?
The isoelectric point, or pI, is the pH at which a molecule carries no net electrical charge, so it does not move in an electric field.
How does a side chain change the pI?
An ionizable side chain shifts which pair of pKa values defines the neutral form. An acidic side chain averages with the carboxyl pKa, while a basic side chain averages with the amino pKa.
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